Thermo Fisher Scientific CK2 alpha-2 Monoclonal Antibody (1E1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E1

Immunogen

Purified recombinant fragment of human CSNK2A2 (amino acids: 194-350) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CK2 alpha-2,uniProtId:P19784-1,ncbiNodeId:9606,antigenRange:194-350,antigenLength:350,antigenImageFileName:MA5-17062_CK2_alpha-2_P19784-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17062_CK2_alpha-2_P19784-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538533

Product Specific Information

MA5-17062 targets CSNK2A2 in FACS, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17062 immunogen is purified recombinant fragment of human CSNK2A2 (amino acids: 194-350) expressed in E. Coli.

MA5-17062 detects CSNK2A2 which has a predicted molecular weight of approximately 41.2kDa.

Target Information

Casein kinase II is a protein serine/threonine kinase that is thought to be present in all eukarotic cells, implying that it has fundamental cellular functions. The holoenzyme is a tetramer containing 2 alpha or alpha-prime subunits (or one of each) and 2 beta subunits. The function of beta subunits is unknown and the alpha subunit is the catalytic unit. CK2 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha) in a homomeric or heteromeric conformation. Whilst the alpha- and alpha-subunits are catalytically identical, proteins that regulate CK2, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha`-subunit. CK2 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CK2 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.