Thermo Fisher Scientific DKK3 Monoclonal Antibody (8A5C6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8A5C6

Immunogen

Purified recombinant fragment of human DKK3 (amino acids: 91-350 ) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DKK3,uniProtId:Q9UBP4-1,ncbiNodeId:9606,antigenRange:91-350,antigenLength:350,antigenImageFileName:MA5-17067_DKK3_Q9UBP4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17067_DKK3_Q9UBP4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538538

Product Specific Information

MA5-17067 targets DKK3 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17067 immunogen is purified recombinant fragment of human DKK3 (amino acids: 91-350 ) expressed in E. Coli.

MA5-17067 detects DKK3 which has a predicted molecular weight of approximately 38.3kDa.

Target Information

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.