Thermo Fisher Scientific LGMN Monoclonal Antibody (312114)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

312114

Immunogen

NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase Ile18-Tyr433 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LGMN,uniProtId:Q99538-1,ncbiNodeId:9606,antigenRange:18-433,antigenLength:433,antigenImageFileName:MA5-24032_LGMN_Q99538-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24032_LGMN_Q99538-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605859

Product Specific Information

In direct ELISAs, no cross-reactivity with recombinant mouse Legumain is observed. This antibody recognizes the pro form of human Legumain and does not recognize the mature form (aa 18-323).

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.