Thermo Fisher Scientific HIF3A Monoclonal Antibody (OTI2F7), TrueMAB
인간 HIF3A 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 적합합니다. 동결건조 형태로 제공되며, PBS 및 trehalose 버퍼에 저장됩니다. 연구용으로만 사용 가능하며, 재구성 및 탈염 후 결합 실험에 활용할 수 있습니다.
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Thermo Fisher Scientific HIF3A Monoclonal Antibody (OTI2F7), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution
- 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 355–669 of human HIF3A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Cell growth and viability are compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors such as HIF-1α, HIF-1β (Arnt 1), EPAS-1 (HIF-2α), and HIF-3α promote glycolysis, erythropoiesis, and angiogenesis to restore oxygen homeostasis.
These factors belong to the Per-Arnt-Sim (PAS) domain transcription factor family. Under hypoxic conditions, HIF-1α forms a heterodimer with Arnt 1 to generate the HIF-1 complex, which binds to hypoxia-responsive elements (HRE) in target genes to activate transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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