Thermo Fisher Scientific MGMT Monoclonal Antibody (OTI4D5), TrueMAB
Human MGMT 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot에 최적화됨. 무보존제, 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. DNA 손상 복구 연구 및 항암제 내성 메커니즘 분석에 적합.
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Applications
- Western Blot (WB)
Tested Dilution
- 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D5 |
| Immunogen | Full length human recombinant protein of human MGMT produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a key DNA repair protein that confers resistance to chemotherapeutic alkylating agents in tumor cells. It protects normal cells from genotoxic and carcinogenic effects of DNA alkylation by removing alkyl groups from the O6 position of guanine. MGMT expression levels vary widely depending on cell type, tissue, species, and growth conditions, as well as among and within tumor types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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