Thermo Fisher Scientific ATM Monoclonal Antibody (M361)

Thermo Fisher Scientific ATM Monoclonal Antibody (M361)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects a 370 kDa protein corresponding to the molecular mass of ATM on SDS-PAGE immunoblots of human A431 and Jurkat cells.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer.

The ATM gene encodes a member of the phosphoinositide 3-kinase (PI3K) superfamily. Unlike lipid kinases, ATM phosphorylates proteins and regulates cell-cycle checkpoints via downstream targets such as p53, Mdm2, BRCA1, and SMC1.

ATM plays a critical role in repairing double-stranded DNA breaks induced by ionizing radiation and other mutagens. The C-terminal region of ATM shares homology with catalytic domains of PI3 kinases. ATM becomes autophosphorylated and activated upon exposure to ionizing radiation.

AT cells are hypersensitive to ionizing radiation, exhibit impaired DNA synthesis inhibition, and show delayed p53 induction. DNA damage activates ATM kinase, initiating a cascade of reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have also linked ATM to apoptosis regulation via Nbs1 and Chk2 in the E2F1 pathway.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.