Thermo Fisher Scientific CD55 Monoclonal Antibody (M002)

Applications

Product Specifications

Product Specific Information

Clone M002 detects 60–80 kDa bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of native A431, A549, and MDA-MB-231 cell lysates.
The antibody also detects a native recombinant human CD55 extracellular region but does not detect the denatured form of CD55.
It is suitable for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.

Target Information

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects cells from complement cascade activation.
Interactions between CD55 and CD97 are involved in T cell regulation. CD55 promotes neoangiogenesis, tumorigenesis, invasiveness, and apoptosis evasion in tumors.
It is a 70 kDa glycoprotein anchored by a glycosylphosphatidylinositol tail, with variable molecular weight depending on cell type and glycosylation.
CD55 binds to activated C4b or C3b complement fragments, preventing assembly and accelerating decay of both classical and alternative pathways.
It also carries Cromer-related blood group antigens.
Two alternatively spliced transcripts exist: one encoding a membrane-bound form and another encoding a soluble form expressed at lower levels.
CD55 has been studied for its role in prostate cancer growth and survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.