Thermo Fisher Scientific Protein APC Monoclonal Antibody (KT45)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

Immunocytochemistry (ICC/IF)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KT45

Immunogen

Clone KT45 was generated from a recombinant protein containing amino acid residues from the C-terminal region of mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2942676

Product Specific Information

This antibody detects a primarily full length APC at 300 kDa* in human Hct116 cells, mouse brain, and in rat PC12 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.

Target Information

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.