Thermo Fisher Scientific IFN gamma Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines. It is a dimeric glycoprotein composed of two 146 amino acid subunits with a molecular weight of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation. The biological activity of IFN gamma is species specific; human IFN gamma does not cross-react with mouse. Functions include antiviral and antiproliferative activities, induction of MHC class I/II, macrophage activation, and enhancement of immunoglobulin secretion by B lymphocytes. IFN gamma acts via IFN gamma receptor I and II, activating the JAK-STAT pathway. It shares about 40% sequence homology with mouse IFN gamma and is regulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.