Thermo Fisher Scientific IFN gamma Polyclonal Antibody
Thermo Fisher Scientific의 IFN gamma Polyclonal Antibody는 Rat IFNγ에 반응하는 Rabbit IgG 항체로, WB, IHC, ELISA에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며 재구성 후 500 µg/mL 농도 유지. 연구용으로만 사용.
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Thermo Fisher Scientific IFN gamma Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived rat IFN gamma recombinant protein (Position: 23–156) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2884874 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.
- IFN gamma is a dimeric glycoprotein consisting of two 146 amino acid subunits.
- Functionally exists as a homodimer (~45 kDa).
- On SDS-PAGE, appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
- Biological activity is highly species-specific; human IFN gamma does not cross-react with mouse.
- Functions include antiviral and tumor antiproliferative activity, induction of MHC class I/II, macrophage activation, and enhanced B lymphocyte immunoglobulin secretion.
- Acts synergistically with other cytokines and activates the JAK-STAT pathway via IFN gamma receptor I and II.
- Human IFN gamma shares ~40% sequence homology with mouse IFN gamma.
- Upregulated by IL2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
- Gene mutations associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-114444_IFN_gamma_P01581-1_Rabbit.svg, PA5-114444_IFN_gamma_P01581-1_Rabbit_PDP.jpeg)
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