Thermo Fisher Scientific CXCL2 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 3 publications 3 publications

In vitro Assay (IV)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine MIP-2 (CXCL2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL2,uniProtId:P10889-1,ncbiNodeId:10090,antigenRange:28-100,antigenLength:100,antigenImageFileName:500-P130-100UG_CXCL2_P10889-1_Rabbit.svg,antigenImageFileNamePDP:500-P130-100UG_CXCL2_P10889-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929381

Product Specific Information

AA Sequence of recombinant protein: AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-2 (CXCL2). Anti-Murine MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography employing an immobilized Murine MIP-2 (CXCL2) matrix.

Sandwich ELISA: To detect Murine MIP-2 (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine MIP-2 (CXCL2) (500-P130Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-2 (CXCL2).

Western Blot: To detect Murine MIP-2 (CXCL10) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-2 (CXCL10) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.