Thermo Fisher Scientific CXCL2 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine MIP-2 (CXCL2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL2,uniProtId:P10889-1,ncbiNodeId:10090,antigenRange:28-100,antigenLength:100,antigenImageFileName:500-P130BT-500UG_CXCL2_P10889-1_Rabbit.svg,antigenImageFileNamePDP:500-P130BT-500UG_CXCL2_P10889-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-2 (CXCL2). Anti-Murine MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine MIP-2 (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-2 (CXCL2) (500-P130) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-2 (CXCL2).

Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P130BT-1MG will be provided as 2 x 500 µg

Target Information

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.