Thermo Fisher Scientific CXCL11 Polyclonal Antibody, PeproTech
CXCL11 단백질을 인식하는 토끼 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, ELISA, Immunostaining에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로 I-TAC(CXCL11) 검출에 최적화된 고순도 항체입니다.
- 카탈로그번호
- 500-P132-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL11 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Immunostaining (IS) | - | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human I-TAC (CXCL11) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929395 |
Product Specific Information
AA Sequence of recombinant protein:
FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human I-TAC (CXCL11). Anti-Human I-TAC (CXCL11)-specific antibody was purified by affinity chromatography employing an immobilized Human I-TAC (CXCL11) matrix.
Sandwich ELISA:
To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human I-TAC (CXCL11) (500-P132Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human I-TAC (CXCL11).
Western Blot:
To detect Human I-TAC (CXCL11) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human I-TAC (CXCL11) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interferon-inducible T-cell alpha chemoattractant (I-TAC), belonging to the CXC chemokine family, is expressed in peripheral blood leukocytes, pancreas, liver, thymus, spleen, lung, small intestine, placenta, and prostate.
Its molecular weight is 8–11 kD. I-TAC is also known as CXCL-11 and IP-9.
The cytokine regulates calcium release and induces a chemotactic response in activated T-cells, serving as the dominant ligand for CXC receptor-3.
The gene encoding this protein contains 4 exons and at least three polyadenylation signals, reflecting cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene.
It is upregulated in patients with AIDS, dementia, and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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