Thermo Fisher Scientific IL-13 Polyclonal Antibody, PeproTech
Human IL-13을 인식하는 Rabbit Polyclonal Antibody로, Western Blot, ELISA, IHC 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 면역조절 및 알레르기 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-13 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929387 |
Product Specific Information
AA Sequence of Recombinant Protein:
MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGRFN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-13. The anti-Human IL-13 antibody was purified by affinity chromatography using an immobilized Human IL-13 matrix.
Sandwich ELISA:
For detecting Human IL-13 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is recommended. In conjunction with PeproTech Biotinylated Anti-Human IL-13 (500-P13Bt) as a detection antibody, this allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-13.
Western Blot:
For detecting Human IL-13 by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-13 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity and inhibiting production of pro-inflammatory cytokines and chemokines. IL-13 plays a role in allergen-induced asthma, independent of IgE and eosinophils, and regulates B cell maturation, differentiation, CD23 and MHC class II expression, and promotes IgE isotype switching. Structurally, IL-13 resembles IL-3, IL-4, IL-5, and GM-CSF. The IL-13 gene is located on chromosome 5q within a cytokine gene cluster close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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