Thermo Fisher Scientific IL-13 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

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Immunohistochemistry (IHC)

-

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ELISA (ELISA)

0.5-2.0 µg/mL

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Neutralization (Neu)

-

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In vitro Assay (IV)

-

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Immunostaining (IS)

-

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929387

Product Specific Information

AA Sequence of recombinant protein: MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGRFN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-13. Anti-Human IL-13-specific antibody was purified by affinity chromatography employing an immobilized Human IL-13 matrix.

Sandwich ELISA: To detect Human IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-13 (500-P13Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-13.

Western Blot: To detect Human IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.