Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine VEGF 165. The anti-Murine VEGF 165-specific antibody was purified by affinity chromatography using an immobilized Murine VEGF 165 matrix.

Sandwich ELISA:
To detect Murine VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine VEGF 165 (500-P131Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine VEGF 165.

Western Blot:
For detection of Murine VEGF 165 by Western blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine VEGF165 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are fundamental regulators of angiogenesis signaling. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine associated with this process. Five isoforms of VEGF are generated through alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which are expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.