Thermo Fisher Scientific VCAM-1 (CD106) Recombinant Rabbit Monoclonal Antibody (SA05-04)
Recombinant rabbit monoclonal antibody against VCAM-1 (CD106). Validated for WB, IHC, ICC/IF in multiple species. Offers high specificity, sensitivity, and lot-to-lot consistency. Supplied as liquid, 1 mg/mL, stored at 4°C or -20°C.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2000–1:5000 | View 5 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 | – |
| Immunohistochemistry (Frozen) (IHC-F) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:1000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA05-04 |
| Immunogen | Synthetic peptide within Human VCAM1 aa 690–739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809259 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Key advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the larger rabbit immune repertoire
Target Information
VCAM-1 (Vascular Cell Adhesion Molecule 1) is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may play a role in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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