Thermo Fisher Scientific GFAP Recombinant Rabbit Monoclonal Antibody (SA03-04)
GFAP 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. WB, IHC, ICC/IF 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공. HEK293 세포 발현 시스템을 이용해 생산된 고품질 항체로 신경세포 및 뇌종양 연구에 유용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA03-04 |
| Immunogen | Synthetic peptide within Human GFAP aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809263 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed primarily in astrocytes and certain astroglia of the central nervous system. It is also found in satellite cells of peripheral ganglia, non-myelinating Schwann cells, neural stem cells, and various tissues such as lens epithelium and liver Kupffer cells.
GFAP antibodies serve as markers for astrocytic cells and are useful in distinguishing astrocytes from other glial cells during development. Mutations in GFAP cause Alexander disease, a rare astrocytic disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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