Thermo Fisher Scientific Phospho-ZAK (Ser165) Polyclonal Antibody
ZAK 단백질의 Ser165 인산화 상태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 ICC/IF에 적합하며, Human, Mouse, Rat 시료에 반응. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP3K20 (Accession Q9NYL2), corresponding to amino acid residues around phosphorylated Ser165. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817323 |
Product Specific Information
This antibody detects endogenous levels of ZAK only when phosphorylated at Ser165.
Target Information
ZAK is a leucine zipper-containing mitogen-activated protein related to the MLKs in both sequence and function. Like the MLKs and other MAP3Ks, ZAK activates MAP2Ks. ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and plays a role in cell cycle checkpoint regulation. It also has pro-apoptotic activity. Alternate transcriptional splice variants encoding different isoforms have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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