Thermo Fisher Scientific Phospho-TEK (Ser1119) Polyclonal Antibody
인간 및 생쥐 TEK 단백질의 인산화 Ser1119 부위를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 Immunocytochemistry에 사용 가능. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human TEK (Accession Q02763), corresponding to amino acid residues around phosphorylated Ser1119. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817321 |
Product Specific Information
This antibody detects endogenous levels of TIE2 only when phosphorylated at Ser1119.
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling is critical for endothelial cell–smooth muscle cell communication during venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway essential for embryonic vascular development. Immunoblotting studies show that TIE2 expression is increased by thyroid-stimulating hormone and agents that elevate intracellular cAMP. Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent and antiapoptotic, comprising a subpopulation that adheres to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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