Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), DyLight 680
MEK2 단백질의 C-말단을 인식하는 Mouse 단클론 항체로 DyLight 680 형광 표지. Western blot 및 ELISA에 적합하며 인간, 마우스, 랫트 반응성. 단백질 A 정제, 안정적 저장 및 고감도 신호 검출 제공.
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Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), DyLight 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A6.G1.G11 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only before immediate use.
Reacts specifically with human MEK2. Cross-reactivity expected with human, mouse, and rat based on sequence identity of the peptide immunogen. Does not react with MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. MEK2 activates ERK1/2 and MAPK enzymes by phosphorylating threonine/glutamate/tyrosine residues in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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