Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), DyLight 800

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Atto 550
• Atto 647N
• Biotin
• Cyanine3
• Cyanine5
• DyLight 405
• DyLight 488
• DyLight 549
• DyLight 649
• DyLight 680
• HRP
• Custom conjugation available upon request

Product Specific Information

• Store vial at 4°C prior to reconstitution. For extended storage, aliquot and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if solution is not clear after standing.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody specifically recognizes the 6X His motif and is useful for detecting His-tagged proteins in various assays.
It reacts with all tested His-tagged proteins and does not cross-react with endogenous bacterial proteins.
Recognizes His-tag (His-His-His-His-His-His) fused to either the N- or C-terminus of target proteins in transfected or transformed cells.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal of recombinant proteins to facilitate purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody exists.
His-tagged proteins can be purified using Ni²⁺-NTA affinity chromatography due to the strong and selective binding between histidine residues and nickel ions.
His tag-specific antibodies are widely used for detection, purification, and co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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