Thermo Fisher Scientific IL-21 Polyclonal Antibody
Thermo Fisher Scientific IL-21 Polyclonal Antibody는 마우스 IL-21을 검출하기 위한 염기성 다클론 항체로, WB, ICC/IF, ELISA, Neutralization 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 높은 특이성과 낮은 교차 반응성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 0.2–0.8 µg/mL |
| Neutralization (Neu) | 0.2–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant mouse IL-21 (Pro25–Ser146) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605560 |
Product Specific Information
- In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse IL-2, IL-4, IL-15, and recombinant human IL-21 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.30 EU per 1 µg of the antibody (LAL method).
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine mainly produced by Natural Killer T (NKT) cells, T helper (Th17), and T follicular helper (TFH) cells.
In TFH cells, IL-21 signals through IL-21R and STAT3, activating transcription via Bcl6.
IL-21 is critical for TFH cell development, B cell differentiation into plasma and memory cells, IgG production, and apoptosis in naive B cells.
In Th17 cells, IL-21 promotes IL-23R expression through STAT3, IRF4, and RORγt, supporting maturation under inflammatory conditions.
IL-21 also downregulates Foxp3 expression. IL-21 deficient mice show protection against chemically induced colitis, while elevated IL-21 levels are observed in colitis models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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