
Thermo Fisher Scientific CXCL1 Polyclonal Antibody
Rat CXCL1 단백질을 인식하는 Goat Polyclonal Antibody로, WB, IHC, Neutralization 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 높은 특이성과 낮은 교차 반응성을 보유. 동결 건조 형태로 제공되며 -20°C에서 보관.
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Thermo Fisher Scientific CXCL1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL |
| Neutralization (Neu) | 0.15–0.75 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant rat CXCL1/GRO alpha/KC/CINC-1 (Ala25–Lys96) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609427 |
Product Specific Information
- In direct ELISAs, less than 50% cross-reactivity with recombinant mouse KC is observed.
- Less than 10% cross-reactivity with recombinant human GRO alpha, GRO beta, GRO gamma, mouse MIP-2, and rat CINC-2 alpha/beta and CINC-3.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: < 0.10 EU per 1 µg of antibody (LAL method).
Target Information
CXCL1, CXCL2, and CXCL3 (also known as GRO alpha, GRO beta, and GRO gamma) are members of the CXC chemokine subfamily.
These proteins bind with high affinity to IL-8 receptor type B and act as potent neutrophil attractants and activators.
Processed forms GRO-alpha(4–73), GRO-alpha(5–73), and GRO-alpha(6–73) exhibit approximately 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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