Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP
Human 및 Mouse 시료에서 NAG-1 단백질을 검출하는 HRP 결합 폴리클로날 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제. 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-NAG-1 (C-terminal specific) antibody prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A cysteine residue was added to facilitate coupling to KLH. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1.07 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.
A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque (100% homology).
Partial reactivity is expected against rat (86% homology).
Cross-reactivity with other species has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, which plays important roles in prenatal development, postnatal growth, and tissue remodeling.
It is predominantly expressed in the placenta and at lower levels in other tissues.
Presence in amniotic fluid and elevated serum levels in pregnant women suggest involvement in gestation and embryonic development.
GDF-15 generally exerts tumor-suppressive activities and is induced via the p53 pathway.
Serum levels are positively correlated with progression of several tumor types, including colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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