Thermo Fisher Scientific beta Actin Polyclonal Antibody, HRP
HRP로 결합된 beta Actin 폴리클로날 항체로 Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 광범위한 종에 반응하며 로딩 컨트롤용으로 활용 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Goat, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a C-terminal region near amino acids 350–375 of human beta actin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid; dilute only prior to immediate use.
This HRP-conjugated beta actin antibody is directed against human beta actin protein.
BLAST analysis indicates broad reactivity with beta actin from various vertebrates and some yeast species, making it an excellent loading control.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein involved in cell motility, structure, and integrity. Beta and gamma actins coexist in most cell types as cytoskeletal components.
Actins polymerize from globular (G-actin) to filamentous (F-actin) forms, forming a two-stranded helix structure.
Due to its ubiquitous expression in eukaryotic cells, beta actin is widely used as a loading control in protein detection assays such as Western blotting.
Antibodies to beta actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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