Thermo Fisher Scientific NAG-1 Polyclonal Antibody, HRP
Human NAG-1(GDF15) 단백질을 인식하는 HRP 결합 토끼 폴리클로날 항체. Western blot, ELISA, IP에 사용 가능. 고순도 친화 크로마토그래피 정제. 4°C 단기, -20°C 장기 보관 권장. 연구용 전용.
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Applications
| Application | Tested Dilution | Note |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody specifically reacts with a D variant sequence of human NAG-1 protein from human tissues. A BLAST analysis suggests partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, which plays an important role during prenatal development, postnatal growth, and tissue remodeling. GDF-15 is expressed predominantly in the placenta and, to a lesser extent, in various other tissues. Its presence in amniotic fluid and elevated serum levels in pregnant women suggest involvement in gestation and embryonic development. GDF-15 generally exhibits tumor-suppressive activity and is produced in response to activation of the p53 pathway. Serum GDF-15 levels correlate positively with neoplastic progression in several tumor types, including colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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