Thermo Fisher Scientific Phospho-Tropomyosin-1 alpha (Ser283) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ELISA (ELISA)

1: 10,000

-

Immunoprecipitation (IP)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Tropomyosin-1 alpha pS283 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding S283 human Tropomyosin-1 alpha pS283. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TPM1,uniProtId:P09493-1,ncbiNodeId:9606,antigenRange:283,antigenLength:284,antigenImageFileName:600-401-J52_TPM1_P09493-1_Rabbit.svg,antigenImageFileNamePDP:600-401-J52_TPM1_P09493-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Tropomyosin-1 alpha pS283 is directed against the phosphorylated form of humand S283 residue. A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with Tropomyosin-1 alpha pS283 from other sources has not been determined.

Target Information

Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.