Thermo Fisher Scientific alpha Tubulin Recombinant Rabbit Monoclonal Antibody (RM113)
Recombinant rabbit monoclonal antibody recognizing alpha Tubulin. Validated for WB, ICC/IF, IP, and ChIP. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for human, mouse, and rat samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
| ChIP assay (ChIP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM113 |
| Immunogen | A peptide corresponding to the C-terminus of alpha-Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744943 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Alpha Tubulin forms a heterodimer with beta Tubulin, each approximately 55 kDa, serving as the fundamental building block of microtubules. These structures are essential for maintaining cell shape, intracellular transport, and mitosis in eukaryotic cells.
Microtubules are composed mainly of alpha and beta tubulins, while gamma tubulin is crucial for microtubule nucleation. Multiple alpha and beta tubulin genes are highly conserved across species. The alpha tubulin gene shows high similarity to the mouse and rat Tuba1 gene, with expression predominantly in differentiated neurological cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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