Thermo Fisher Scientific alpha Tubulin Recombinant Rabbit Monoclonal Antibody (RM113), Biotin
Recombinant rabbit monoclonal antibody recognizing alpha Tubulin with biotin conjugation. Validated for WB, IHC, ICC/IF, IP, and ChIP. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for diverse species including human, mouse...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
| ChIP assay (ChIP) | 1:200 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM113 |
| Immunogen | A peptide corresponding to the C-terminus of alpha-Tubulin |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744942 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
Alpha Tubulin forms a heterodimer with beta Tubulin, each approximately 55 kDa in molecular weight. These heterodimers are the fundamental building blocks of microtubules, essential components of the eukaryotic cytoskeleton.
Microtubules provide structural support, facilitate intracellular transport, and play a key role in mitosis.
Multiple alpha and beta tubulin genes exist and are highly conserved among species. The alpha tubulin gene is similar to the mouse and rat Tuba1 gene, with expression predominantly in differentiated neuronal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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