Thermo Fisher Scientific Aurora B Recombinant Rabbit Monoclonal Antibody (RM278)
Aurora B 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 높은 특이성과 민감도를 제공하며, 로트 간 일관성이 우수합니다. 단백질 A로 정제된 액상 항체로 PBS/BSA/glycerol buffer에 보관합니다.
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Thermo Fisher Scientific Aurora B Recombinant Rabbit Monoclonal Antibody (RM278)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM278 |
| Immunogen | A peptide corresponding to the N-terminus of human Aurora kinase B (UniProt ID: Q96GD4-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744944 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora B (AURKB) localizes to microtubules near kinetochores, specifically to K-fibers, and functions in chromosome alignment, separation, histone modification, and cytokinesis.
The chromosomal passenger complex (CPC) includes Aurora B, INCENP, survivin, and borealin, which together regulate activity, localization, and stability.
Aurora B is overexpressed in several tumors, including breast and colon cancers, and may interact with Aurora-A kinase through feedback mechanisms.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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