Thermo Fisher Scientific alpha-Cardiac Actin Recombinant Rabbit Monoclonal Antibody (RM257)
Recombinant rabbit monoclonal antibody recognizing alpha-cardiac actin. Validated for WB and IHC-P with high specificity and consistency. Produced in HEK293 cells, purified via Protein A, and supplied in PBS with BSA and glycerol. Suitable for research...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM257 |
| Immunogen | Synthetic peptide corresponding to the N-terminus of human alpha-cardiac actin (UniProt ID: P68032-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744941 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Key advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Gene: ACTC1
Actins are highly conserved proteins involved in various types of cell motility. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) as a two-stranded helix. The alpha actins are found in muscle tissues and are major components of the contractile apparatus.
Defects in ACTC1 are associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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