Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (5C6), PE, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (5C6), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The 5C6 monoclonal antibody reacts with human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, and some CD4⁺/CD8⁺ T cells.
Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and can costimulate multiple NK activation receptors.
The counter-receptor for human NKG2D is MICA/MICB, expressed on epithelial tumors including lung, breast, kidney, ovary, prostate, and colon carcinoma.
5C6 and 1D11 block binding of soluble MICA to γδ TCR T cell clones and inhibit lysis by these cells. They also induce NKG2D-mediated redirected lysis of Fc receptor-bearing P815 cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells.
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Natural killer (NK) cells mediate lysis of certain tumor and virus-infected cells without prior activation.
They regulate humoral and cell-mediated immunity and express calcium-dependent (C-type) lectins involved in NK cell function.
The NKG2 gene family encodes type II transmembrane proteins with extracellular C-termini and C-type lectin domains.
This family is located within the NK complex, a region containing multiple C-type lectin genes preferentially expressed in NK cells.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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