Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein with an immunoglobulin and mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic region. It is expressed selectively by differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells, but absent on Th2 and Tc2 cells. Naive T cells, B cells, macrophages, and dendritic cells do not express CD366 at the protein level.

Expression of CD366 increases at a late stage of T cell differentiation, suggesting a role in Th1 effector function rather than differentiation. In experimental autoimmune encephalomyelitis (EAE) models, CD366 is expressed on most CNS-infiltrating T cells at disease onset, while peripheral expression remains low. Administration of 8B.2C12 in vivo induces a hyperacute, atypical disease phenotype, implicating CD366 in macrophage activation and autoimmune severity regulation. The Tim gene family may play a key role in autoimmunity and allergy regulation.

The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366, with weaker reactivity to the C57Bl/6 allele.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and Tim-3 transfected cells. Recommended ≤0.06 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2) is a 281-amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It contains an IgV-like domain, a mucin-like extracellular domain, and a cytoplasmic tyrosine phosphorylation motif. TIM3 is involved in macrophage activation, autoimmune induction, and immune tolerance regulation. It modulates Th1-mediated immune responses and contributes to the severity of experimental autoimmune encephalomyelitis (EAE) in mice.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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