Thermo Fisher Scientific Phospho-LCK (Tyr505) Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:2,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of LCK only when phosphorylated at Tyr505.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR activation, LCK phosphorylates ITAM motifs in the zeta subunits, creating binding sites for ZAP70 SH2 domains. ZAP70 is subsequently phosphorylated and activated by LCK, initiating downstream signaling via adaptor LAT phosphorylation.

Most LCK is localized to the plasma membrane, but a portion associates with the Golgi apparatus, possibly contributing to Raf activation under weak TCR stimulation. LCK possesses N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein-protein interactions. It also participates in regulating apoptosis induced by various stimuli, excluding death receptor pathways.

Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splicing generates multiple isoforms of the LCK protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.