Thermo Fisher Scientific Phospho-LCK (Tyr394) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of Lck only when phosphorylated at Tyr394.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in zeta subunits, creating binding sites for SH2 domains of ZAP70, which is subsequently phosphorylated and activated by LCK. This activation leads to downstream signaling via adaptor LAT.

Most LCK is localized to the plasma membrane, with a fraction associated with the Golgi apparatus that may contribute to Raf activation under weak TCR stimulation. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2/SH3 domains mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs. It also plays roles in regulating apoptosis induced by various stimuli, excluding death receptor pathways.

Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.