Thermo Fisher Scientific CD47 Monoclonal Antibody (M054)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Clone M054 mouse monoclonal antibody detects native human CD47, as well as aldehyde-fixed CD47.
This antibody is suitable for staining in immunocytochemistry, capture and detection in ELISA, native immunoprecipitation, and western blot.
It detects a 45–55 kDa band in native western blots of A431, BEAS-2B, MCF7, NCI-H446, NCI-H28, and NCI-H2052 cell lines.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions.
Engagement of CD47 by soluble ligands or counter receptors modulates signaling pathways, such as activation of heterotrimeric G proteins.
Functionally, CD47 is involved in increasing intracellular calcium concentration upon cell adhesion to the extracellular matrix.
It also serves as a receptor for the C-terminal cell-binding domain of thrombospondin and may play roles in membrane transport and signal transduction.
CD47 has broad tissue distribution but is reduced on Rh erythrocytes.
It acts as a ligand for CD172a (signal regulatory protein alpha, SIRPα), an immune inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells.
The CD47–CD172a system affects cell migration, B cell adhesion, T cell activation, and modulates chondrocyte responses to mechanical signals, promoting neuronal development, especially in synapse-rich regions of the brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.