Thermo Fisher Scientific CD244 (2B4) Monoclonal Antibody (M085)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Clone M085 mouse monoclonal antibody detects a 110 kDa protein on SDS-PAGE native immunoblots of human THP1 monocytes.
The antibody detects only the native CD244 protein and the aldehyde-fixed CD244 protein.
It is suitable for native western blot, immunoprecipitation, immunocytochemistry, and ELISA capture applications.

Target Information

CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor.
It contains two extracellular Ig-like domains and a cytoplasmic domain with four immunoreceptor tyrosine-based switch motifs.
CD244 binds CD48, a transmembrane receptor expressed on hematopoietic cells, and its activity is regulated by adapter proteins SH2D1A/SAP and SH2D1B/EAT-2.
Downstream signaling involves VAV1, INPP5D/SHIP1, and CBL, leading to NK cell cytotoxicity, IFN-γ production, and granule exocytosis.
CD244 also regulates CD8+ T-cell proliferation and modulates inflammatory responses in dendritic cells.
In cancers, CD244 expression increases in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may enhance T-cell infiltration and inhibit tumor growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.