Thermo Fisher Scientific Phospho-EGFR (Tyr1173) Recombinant Rabbit Monoclonal Antibody (SC57-04)
EGFR Tyr1173 인산화 부위를 인식하는 recombinant rabbit monoclonal antibody. Western blot, IHC(P), ICC/IF에 적합. 높은 특이성과 재현성 제공. 단기간 4°C, 장기간 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC57-04 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of Human EGFR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809498 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and the best clones are selected for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression is associated with multiple cancers, making EGFR a therapeutic target for biological and small molecule inhibitors.
EGFR is encoded by the EGFR gene on chromosome 7 and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4. Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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