Thermo Fisher Scientific Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody (SC65-06)
Cytokeratin 14을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 단백질 A 정제 및 동물 유래 성분이 없는 제형입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC65-06 |
| Immunogen | Recombinant protein within mouse cytokeratin 14 aa 250–484 / 484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809501 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, found in both keratinizing and non-keratinizing tissues. At least 20 different CKs are known, classified as type I (acidic polypeptides) or type II (basic polypeptides).
Keratin 14, a type I low molecular weight keratin, forms a pair with keratin 5 and is a marker distinguishing stratified epithelial cells from simple epithelial cells. It is useful for identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinomas. Both acidic and basic keratins are expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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