Thermo Fisher Scientific WT1 Recombinant Rabbit Monoclonal Antibody (SC06-41)
Thermo Fisher Scientific의 WT1 Recombinant Rabbit Monoclonal Antibody (SC06-41)는 인간 및 마우스 WT1 단백질을 검출하기 위한 고특이성 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 재조합 기술로 생산되어 로트 간 일관성과 높은 민감도를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC06-41 |
| Immunogen | Recombinant protein within Human WT1 (aa 59–269) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet Ice |
| RRID | AB_2809502 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production clones.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and nuclear envelope reassembly.
Arabidopsis thaliana RanGAP association with the nuclear envelope involves nucleoporins with coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). WT1 and WIT2 form a second family of CC-TMD proteins distinct from WIPs, necessary for RanGAP nuclear envelope association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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