Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
p27 Kip1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 세포 주기 조절 연구에 유용합니다. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human p27 Kip1 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: p27 Kip1.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and is proposed to mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression, acting as a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes.
p27 Kip1 also forms complexes with cyclin D-CDK4, contributing to the assembly, stability, and modulation of CCND1-CDK4 activation. Its function as an inhibitor or activator depends on phosphorylation state and stoichiometry.
This 27 kDa protein undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, a process essential for the transition from quiescence to proliferation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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