Thermo Fisher Scientific p27 Kip1 Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 p27 Kip1 폴리클로날 항체로 Western blot 및 IHC에 사용 가능. 합성 펩타이드를 면역원으로 하며, 동결건조 형태로 제공. 세포 주기 조절 단백질 p27 Kip1 검출에 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human p27 Kip1 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: p27 Kip1.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and is proposed to mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 interacts with cyclin D-CDK4 complexes, influencing their assembly, stability, and activation. Its activity depends on phosphorylation state and stoichiometry. The protein has a molecular weight of approximately 27 kDa, and its degradation—triggered by CDK-dependent phosphorylation and ubiquitination by SCF complexes—is essential for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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