Thermo Fisher Scientific ATF1 Monoclonal Antibody (OTI2C2), TrueMAB
ATF1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot과 IHC(Paraffin)에 적합합니다. 정제된 항체로, 동결건조 형태이며 PBS buffer에 8% trehalose를 포함합니다. 연구용으로 세포 신호전달 및 전사 조절 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C2 |
| Immunogen | Full length human recombinant protein of human ATF1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ATF1 gene encodes an activating transcription factor belonging to the ATF subfamily and bZIP family. It regulates downstream target genes involved in cell growth, survival, and other physiological processes.
Phosphorylation at serine 63 by kinases such as PKA, CaMK I/II, MSK, and CDK3 enhances its transcriptional activity and promotes cell transformation.
Chromosomal translocations involving ATF1 and FUS (chromosome 16) or EWSR1 (chromosome 22) produce chimeric proteins associated with angiomatoid fibrous histiocytoma and clear cell sarcoma.
A pseudogene is located on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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