Thermo Fisher Scientific PELI1 Monoclonal Antibody (OTI13F8), TrueMAB
인간 PELI1 단백질을 인식하는 단일클론 항체로, Western blot과 IHC(파라핀 절편)에 사용 가능. E3 유비퀴틴 리가아제 연구에 적합하며, 동결건조 형태로 제공. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 보장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI13F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-245 of human PELI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Pellino proteins are a family of highly homologous E3 ubiquitin ligases that function as upstream mediators in Toll-like receptor (TLR) signaling pathways, leading to MAP kinase and transcription factor activation.
Pellino 1 interacts with the IRAK4–IRAK–TRAF6 complex to mediate IL-1 signaling and activate NF-κB. It catalyzes polyubiquitination of IRAK1 and is regulated by phosphorylation (via IRAK1, IRAK4, IKKε, and TBK1), ubiquitination, and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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