Thermo Fisher Scientific PRMT5 Monoclonal Antibody (OTI2H3), TrueMAB
인간 PRMT5 단백질(aa 238-499)에 특이적인 마우스 단클론 항체로, IHC(P)에서 1:150 희석으로 검증됨. 무보존제, 동결건조 형태이며, PBS/trehalose 버퍼에 저장. 연구용으로만 사용 가능.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 238–499 of human PRMT5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that catalyzes the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for MMA.
It mediates symmetrical dimethylation of arginine residues in small nuclear ribonucleoproteins Sm D1 and Sm D3, which is essential for assembly and biogenesis of snRNP core particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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