Thermo Fisher Scientific SMAD1 Polyclonal Antibody
SMAD1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human과 Rat 시료에 반응합니다. Western blot 및 ELISA에 적합하며, Affinity Chromatography로 정제되었습니다. PBS/glycerol buffer에 보관하며, 세포 내 SMAD 신호전달 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 20–240 of human Smad1 (NP_001003688.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.28 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913679 |
Product Specific Information
- Positive test controls: Jurkat, C6
- Subcellular localization: Cytoplasm, Nucleus
Immunogen sequence:
WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSCPG QPSNCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLECC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHSEYNPQ HSLLAQFRNL GQNEPHMPLN ATFPDSFQQP NSHPFPHSPN SSYPNSPGSS SSTYPHSPTS SDPGSPFQMP ADTPPPAYLP PEDPMTQDGS Q
Target Information
The SMAD1 protein belongs to the SMAD family, homologous to the Drosophila mothers against decapentaplegic (Mad) and C. elegans Sma genes. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD1 mediates bone morphogenetic protein (BMP) signaling, which regulates cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand binding, SMAD1 is phosphorylated and forms a complex with SMAD4 to regulate transcription. It is also targeted by SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) for ubiquitination and proteasomal degradation. Alternatively spliced variants encoding the same protein have been observed.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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