Thermo Fisher Scientific AKT1 Polyclonal Antibody
AKT1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA에 사용 가능. Human, Mouse, Rat 반응성. Affinity Chromatography로 정제되었으며, 액상 형태로 -20°C 보관. 세포 생존 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 350–480 of human AKT1 (NP_0051542) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913680 |
Product Specific Information
- Positive test controls include: MCF7
- Subcellular localization: Cell membrane, Cytoplasm, Nucleus
Immunogen sequence:
YNQDHEKLFE LILMEEIRFP RTLGPEAKSL LSGLLKKDPK QRLGGGSEDA KEIMQHRFFA GIVWQHVYEK KLSPPFKPQV TSETDTRYFD EEFTAQMITI TPPDQDDSME CVDSERRPHF PQFSYSASGT A
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating multiple targets in various cell types, including cancer cells. This kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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