Thermo Fisher Scientific Vinculin Recombinant Rabbit Monoclonal Antibody (9I3Y8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300,000-1:1,280,000

-

Immunocytochemistry (ICC/IF)

1:800-1:3,200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9I3Y8

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_0547061) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vinculin,uniProtId:P18206-1,ncbiNodeId:9606,antigenRange:800-900,antigenLength:1134,antigenImageFileName:MA5-42795_Vinculin_P18206-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42795_Vinculin_P18206-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2911936

Product Specific Information

Positive test controls include: HeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain. The target is usually found in the following locations: Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion.

Immunogen sequence: DAKAVAGNIS DPGLQKSFLD SGYRILGAVA KVREAFQPQE PDFPPPPPDL EQLRLTDELA PPKPPLPEGE VPPPRPPPPE EKDEEFPEQK AGEVINQPMM M

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.