Thermo Fisher Scientific H3K27ac Recombinant Rabbit Monoclonal Antibody (2V10N10)
H3K27ac 표적의 인간 히스톤 H3에 특이적인 재조합 토끼 단클론 항체로, WB, IHC, ICC, ELISA, IP, ChIP 등 다양한 응용에 적합합니다. 높은 민감도와 특이성을 가지며, 액상 형태로 -20°C 보관됩니다.
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Thermo Fisher Scientific H3K27ac Recombinant Rabbit Monoclonal Antibody (2V10N10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:120,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 2 µg antibody for 5–10 µg of chromatin |
| Dot blot (DB) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2V10N10 |
| Immunogen | A synthetic acetylated peptide around K27 of human Histone H3 (P68431) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.50 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911939 |
Product Specific Information
- Positive test controls: HeLa, NIH/3T3, C6
- Immunogen sequence:
MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY
Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications such as methylation, acetylation, and phosphorylation of histone H3 influence chromatin structure and transcriptional activity.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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