Thermo Fisher Scientific GNAS Monoclonal Antibody (OTI13G5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Mutations in the GNAS gene are associated with several disorders, including:

• Pseudohypoparathyroidism types 1a and 1b
• Albright hereditary osteodystrophy
• Pseudopseudohypoparathyroidism
• McCune-Albright syndrome
• Progressive osseous heteroplasia
• Polyostotic fibrous dysplasia
• Certain pituitary tumors

The GNAS gene has a complex imprinted expression pattern, encoding multiple alternatively spliced transcripts with distinct 5′ exons located within differentially methylated regions. Some transcripts show unusual promoter proximity (14 kb apart), and one variant introduces a frameshift resulting in a structurally unrelated isoform. An antisense transcript may regulate imprinting in this region. Mutations lead to pseudohypoparathyroidism type 1a (PHP1a), which exhibits atypical autosomal dominant inheritance requiring maternal transmission for full penetrance.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.